Instrumentation

The lab has a single Flow Cytometer system.

• Manufacturer: BD Biosciences
• Model: FACSCalibur

Users are required to take the “Introduction to Flow Cytometry lecture series” provided by the by UWCCC Flow Lab (cost is $107.04 for UW-Madison faculty and students; NOTE: cost is set by the UWCCC and may change). This is a 3-part class (90 minutes each) that provides a great overview of flow cytometry and instrumentation.

The lab has a single FTIR (Fourier Transform Infrared) spectrometer for the analysis of liquids and solids using the attached diamond ATR accessory.

• Manufacturer: Thermo Scientific
• Model: Nicolet iS10
• Accessory: Smart iTX (diamond ATR – attenuated total reflectance)
• Brochure

The lab has a single Freezer Dryer / Lyophilizer for removal of water from samples.

• Manufacturer: LabConco
• Model: FreezeZone 2.5
• Details

The lab has multiple GC (Gas Chromatography) systems for the analysis of organic compounds in water, solvent, or gas.

• Manufacturer (all): Shimadzu Scientific.

(1) GC #3 – w/ ECD (Electron Capture Detector) and Restek RTX-624 (30m) column or user provided column(s)

(2) GC #4 (for gas analysis) w/ both FID (Flame Ionization Detector) and TCD (Thermo Conductivity Detector); installed with system-specific Packed Columns

(3) GC #5 – w/ FID (Flame Ionization Detector) and a variety of columns to choose from [Stabilwax-DA (15m), Stabilwax-DA (30m), Restek RTX-5 (30m)] or user provided column(s)

Samples must be extracted into a solvent (unless using the Stabilwax column on GC5 for fatty acid analysis – can analyze water samples). A set of calibration standards should be prepared in the same solvent.

The lab has a single GC-MS (Gas Chromatography-Mass Spectrometry) system for the analysis of organics.

• Manufacturer: Shimadzu Scientific
• Model: GC-2020 with QP2010S Mass Spectrometer detector
• Column: Restek RXI-5MS (30m, 0.25mm ID, 0.25um) or user provided column

Samples must be extracted into a solvent. A set of calibration standards should be prepared in the same solvent.

The lab has a single HPLC (High Performance Liquid Chromatography) system for the analysis of a variety of analytes from anions to sugars.

• Manufacturer: Shimadzu Scientific
• Model: AT vp system
• Detectors: Diode Array (UV-Vis); Refractive Index (RI); Fluorescence
• Column(s): Restek Ultra Aqueous C18 (4.6 x 150mm, 5um); Bio-Rad Aminex HPX-87H (300 x 7.8 mm, 9um) or user provided column(s)

All samples must be filtered with a 0.45um filter and in 1.7-2.0mL autosampler vials. A set of calibration standards should be prepared under the same conditions as the samples.

The lab has a single ICP-OES (Inductively Coupled Plasma – Optical Emission Spectrometry) system for the analysis of Metals.

• Manufacturer: Varian
• Model: Vista-MPX ICP-OES

Liquid samples must be filtered using a 0.45um filter, adjusted with concentrated Nitric Acid (HNO3) to pH <2, and brought to the lab in 15mL disposable centrifuge tubes (like THESE). A set of calibration standards should be prepared under the same conditions (pH <2 with HNO3, 15mL tubes).

The lab has a single Micropore Size and Chemisorption Analyzer (aka BET) for gas sorption analysis.

• Manufacturer: Quantachrome
• Model: autosorb IQ
• Brochure

The lab has a single epi Fluorescent microscope for both fluorescence and bright field microscopy.

• Manufacturer: Zeiss
• Model: Imager Z2
• New Camera and Software (July 2022)

The lab has a single Laser Diffraction Particle Size Analyzer.

• Manufacturer: Beckman Coulter
• Model: LS 13 320
• Details

The lab has a single Thermogravimetric Analyzer (TGA).

• Manufacturer: TA Instruments
• Model: TGA 550
• Details