Instrumentation

The EECF has a wide variety of instrumentation for independent use by UW-Madison faculty and students.

To schedule training, please visit the Training Page

To reserve an instrument, please visit the Reservations Page

The lab has a single Micropore Size and Chemisorption Analyzer (aka BET) for gas sorption analysis.

  • Manufacturer: Quantachrome
  • Model: autosorb IQ
  • Brochure

Flow Cytometer in EECF Flow Cytometer - EECF

The lab has a single Flow Cytometer system.

  • Manufacturer: BD Biosciences
  • Model: FACSCalibur

Users are required to take the “Introduction to Flow Cytometry lecture series” provided by the by UWCCC Flow Lab (cost is $107.04 for UW-Madison faculty and students; NOTE: cost is set by the UWCCC and may change). This is a 3-part class (90 minutes each) that provides a great overview of flow cytometry and instrumentation.

The lab has a single Freezer Dryer / Lyophilizer for removal of water from samples.

  • Manufacturer: LabConco
  • Model: FreezeZone 2.5
  • Details

 

The lab has a single FTIR (Fourier Transform Infrared) spectrometer for the analysis of liquids and solids using the attached diamond ATR accessory.

  • Manufacturer: Thermo Scientific
  • Model: Nicolet iS10
  • Accessory: Smart iTX (diamond ATR – attenuated total reflectance)
  • Brochure
GC3 - GC-ECD
GC#3
GC4 - GC-2014 (Gas Analysis)
GC#4
GC5 - GC-FID
GC#5

The lab has multiple GC (Gas Chromatography) systems for the analysis of organic compounds in water, solvent, or gas.

  • Manufacturer (all): Shimadzu Scientific.

(1) GC #3 – w/ ECD (Electron Capture Detector) and Restek RTX-624 (30m) column or user provided column(s)

(2) GC #4 (for gas analysis) w/ both FID (Flame Ionization Detector) and TCD (Thermo Conductivity Detector); installed with system-specific Packed Columns

(3) GC #5 – w/ FID (Flame Ionization Detector) and a variety of columns to choose from [Stabilwax-DA (15m), Stabilwax-DA (30m), Restek RTX-5 (30m)] or user provided column(s)

Samples must be extracted into a solvent (unless using the Stabilwax column on GC5 for fatty acid analysis – can analyze water samples). A set of calibration standards should be prepared in the same solvent.

GC-MS

The lab has a single GC-MS (Gas Chromatography-Mass Spectrometry) system for the analysis of organics.

  • Manufacturer: Shimadzu Scientific
  • Model: GC-2020 with QP2010S Mass Spectrometer detector
  • Column: Restek RXI-5MS (30m, 0.25mm ID, 0.25um) or user provided column

Samples must be extracted into a solvent. A set of calibration standards should be prepared in the same solvent.

HPLC

The lab has a single HPLC (High Performance Liquid Chromatography) system for the analysis of a variety of analytes from anions to sugars.

  • Manufacturer: Shimadzu Scientific
  • Model: AT vp system
  • Detectors: Diode Array (UV-Vis); Refractive Index (RI); Fluorescence
  • Column(s): Restek Ultra Aqueous C18 (4.6 x 150mm, 5um); Bio-Rad Aminex HPX-87H (300 x 7.8 mm, 9um) or user provided column(s)

All samples must be filtered with a 0.45um filter and in 1.7-2.0mL autosampler vials. A set of calibration standards should be prepared under the same conditions as the samples.

ICP

The lab has a single ICP-OES (Inductively Coupled Plasma – Optical Emission Spectrometry) system for the analysis of Metals.

  • Manufacturer: Varian
  • Model: Vista-MPX ICP-OES

Liquid samples must be filtered using a 0.45um filter, adjusted with concentrated Nitric Acid (HNO3) to pH <2, and brought to the lab in 15mL disposable centrifuge tubes (like THESE). A set of calibration standards should be prepared under the same conditions (pH <2 with HNO3, 15mL tubes).

The lab has a single epi Fluorescent microscope for both fluorescence and bright field microscopy.

  • Manufacturer: Zeiss
  • Model: Imager Z2

The lab has a single Laser Diffraction Particle Size Analyzer.

  • Manufacturer: Beckman Coulter
  • Model: LS 13 320
  • Details

The lab has a single Thermogravimetric Analyzer (TGA).

  • Manufacturer: TA Instruments
  • Model: TGA 550
  • Details

 

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Flow Cytometer

Flow Cytometer in EECF Flow Cytometer - EECF

The lab has a single Flow Cytometer system.

  • Manufacturer: BD Biosciences
  • Model: FACSCalibur

Users are required to take the “Introduction to Flow Cytometry lecture series” provided by the by UWCCC Flow Lab (cost is $107.04 for UW-Madison faculty and students; NOTE: cost is set by the UWCCC and may change). This is a 3-part class (90 minutes each) that provides a great overview of flow cytometry and instrumentation.

Fourier Transform Infrared Spectrometer (FTIR)

The lab has a single FTIR (Fourier Transform Infrared) spectrometer for the analysis of liquids and solids using the attached diamond ATR accessory.

  • Manufacturer: Thermo Scientific
  • Model: Nicolet iS10
  • Accessory: Smart iTX (diamond ATR – attenuated total reflectance)
  • Brochure

Freeze Dryer (aka Lyophilizer)

The lab has a single Freezer Dryer / Lyophilizer for removal of water from samples.

  • Manufacturer: LabConco
  • Model: FreezeZone 2.5
  • Details

Gas Chromatography (GC-FID, GC-FID-TCD, GC-ECD)

GC3 - GC-ECD
GC#3
GC4 - GC-2014 (Gas Analysis)
GC#4
GC5 - GC-FID
GC#5

The lab has multiple GC (Gas Chromatography) systems for the analysis of organic compounds in water, solvent, or gas.

  • Manufacturer (all): Shimadzu Scientific.

(1) GC #3 – w/ ECD (Electron Capture Detector) and Restek RTX-624 (30m) column or user provided column(s)

(2) GC #4 (for gas analysis) w/ both FID (Flame Ionization Detector) and TCD (Thermo Conductivity Detector); installed with system-specific Packed Columns

(3) GC #5 – w/ FID (Flame Ionization Detector) and a variety of columns to choose from [Stabilwax-DA (15m), Stabilwax-DA (30m), Restek RTX-5 (30m)] or user provided column(s)

Samples must be extracted into a solvent (unless using the Stabilwax column on GC5 for fatty acid analysis – can analyze water samples). A set of calibration standards should be prepared in the same solvent.

Gas Chromatography-Mass Spectrometry (GCMS)

GC-MS

The lab has a single GC-MS (Gas Chromatography-Mass Spectrometry) system for the analysis of organics.

  • Manufacturer: Shimadzu Scientific
  • Model: GC-2020 with QP2010S Mass Spectrometer detector
  • Column: Restek RXI-5MS (30m, 0.25mm ID, 0.25um) or user provided column

Samples must be extracted into a solvent. A set of calibration standards should be prepared in the same solvent.

High Performance Liquid Chromatography (HPLC)

HPLC

The lab has a single HPLC (High Performance Liquid Chromatography) system for the analysis of a variety of analytes from anions to sugars.

  • Manufacturer: Shimadzu Scientific
  • Model: AT vp system
  • Detectors: Diode Array (UV-Vis); Refractive Index (RI); Fluorescence
  • Column(s): Restek Ultra Aqueous C18 (4.6 x 150mm, 5um); Bio-Rad Aminex HPX-87H (300 x 7.8 mm, 9um) or user provided column(s)

All samples must be filtered with a 0.45um filter and in 1.7-2.0mL autosampler vials. A set of calibration standards should be prepared under the same conditions as the samples.

Inductively Couple Argon Plasma Spectrometer (ICP-OES)

ICP

The lab has a single ICP-OES (Inductively Coupled Plasma – Optical Emission Spectrometry) system for the analysis of Metals.

  • Manufacturer: Varian
  • Model: Vista-MPX ICP-OES

Liquid samples must be filtered using a 0.45um filter, adjusted with concentrated Nitric Acid (HNO3) to pH <2, and brought to the lab in 15mL disposable centrifuge tubes (like THESE). A set of calibration standards should be prepared under the same conditions (pH <2 with HNO3, 15mL tubes).

Micropore Size and Chemisorption Analyzer (aka BET)

The lab has a single Micropore Size and Chemisorption Analyzer (aka BET) for gas sorption analysis.

  • Manufacturer: Quantachrome
  • Model: autosorb IQ
  • Brochure

 

Microscope (epi Fluorescence, Bright Field)

The lab has a single epi Fluorescent microscope for both fluorescence and bright field microscopy.

  • Manufacturer: Zeiss
  • Model: Imager Z2
  • New Camera and Software (July 2022)

 

Particle Size Analyzer

The lab has a single Laser Diffraction Particle Size Analyzer.

  • Manufacturer: Beckman Coulter
  • Model: LS 13 320
  • Details

 

Thermogravimetric Analyzer (TGA)

The lab has a single Thermogravimetric Analyzer (TGA).

  • Manufacturer: TA Instruments
  • Model: TGA 550
  • Details